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24-hour cross-match laboratory

Overview

The 24-hour Cross-Match Laboratory and Red Cell Reference Laboratory make up the immunohaematology section in Blood Services Group (BSG), which provides patient-centric red cells and platelets diagnostic testing services to all hospitals in Singapore.

The 24-hour Cross-Match Laboratory operates round-the clock services for ABO and Rh type determination, pre-transfusion screening for clinically significant red cell antibody and compatibility testing for blood transfusion recipients, as well as issue-specialised blood products to all hospitals.

The Red Cell Reference Laboratory provides specialised referral services to hospitals and clinical laboratories that encounter difficulties in red cell and platelet diagnostic investigation. The investigational services provided range from identification and characterisation of atypical antibody, red cell auto-antibody, cold antibody and resolution of ABO discrepancies as well as platelet antibody screening. The Red Cell Reference Laboratory also runs the National Proficiency Testing Program for ABO Grouping and Rh Typing for participating clinical laboratories in Singapore.

List of immunohaematological tests

Test description Turn-around-time(in working days) Sample requirement Remarks
  • ABO & RH Grouping
  • Red Cell Antibody Screening
  • Red Cell Cross Match
  • Pretransfusion test: up to 3 hours
  • Reference lab: 5 working days
  • 7ml EDTA sample
  • Neonates: 2ml whole blood
  • Fresh sample preferred. If stored, sample should have been at 2 - 8 degrees celsius for not more than 48 hours.
  • For neonatal transfusion (baby is less than 4 months old), mother's sample may be required.
Type and Screen 1
  • 7ml EDTA sample
  • Neonates: 2ml whole blood
  • Fresh sample preferred. If stored, sample should have been at 2 - 8 degrees celsius for not more than 48 hours.
  • For neonatal transfusion (baby is less than 4 months old), mother's sample may be required.
Neonatal ABO & Rh Grouping 5 2ml EDTA sample Fresh sample preferred. If stored, sample should have been at 2 - 8 degrees celsius for not more than 48 hours.
Neonatal Jaundice Profile - NNJ 5 10ml clotted sample (mother) and 1ml EDTA baby sample Fresh sample preferred. If stored, sample should have been at 2 - 8 degrees celsius for not more than 48 hours.
  • Red Cell Allo Antibody Identification
  • Red Cell Antibody ID - Acid Elution
5
  • 10ml clotted sample
  • 5ml EDTA sample
Fresh sample preferred. If stored, sample should have been at 2 - 8 degrees celsius for not more than 48 hours.
Red Cell Auto Antibody Identification 7
  • 10ml clotted sample
  • 5ml EDTA sample
Fresh sample preferred. If stored, sample should have been at 2 - 8 degrees celsius for not more than 48 hours.
  • Red Cell Direct Coombs Test
  • Monospecific Direct Coombs Test
5
  • 5ml EDTA sample
  • Paediatric: 1ml whole blood
Fresh sample preferred. If stored, sample should have been at 2 - 8 degrees celsius for not more than 48 hours.
  • Cold Agglutinin Screen
  • Cold Agglutinin Titration
5 10ml clotted sample Fresh sample preferred. If stored, sample should have been at 2 - 8 degrees celsius for not more than 48 hours.
  • Antibody Titration - Coombs
  • Antibody Titration - ABO (Anti A)
  • Antibody Titration - ABO (Anti B)
5 10ml clotted sample Fresh sample preferred. If stored, sample should have been at 2 - 8 degrees celsius for not more than 48 hours.
Platelet Antibody Screen
  • Batch test: up to 30 working days
  • Urgent cases: within 7 working days
10ml clotted sample Fresh sample preferred. If stored, sample should have been at 2 - 8 degrees celsius for not more than 48 hours.
Red Cell RH Phenotype 5
  • 5ml EDTA sample (preferable)
  • 10ml clotted sample
Fresh sample preferred. If stored, sample should have been at 2 - 8 degrees celsius for not more than 48 hours.
Antigen Typing:
  • Kell
  • Js
  • Kp
  • Kidd
  • Luthens
  • Lewis
  • Duffy
  • Ext Duf
  • MN
  • S
  • P1
5
  • 5ml EDTA sample (preferable)
  • 10ml clotted sample
Fresh sample preferred. If stored, sample should have been at 2 - 8 degrees celsius for not more than 48 hours.
Cryoglubulin Detection (cold 4 degrees celsius) 7
  • 10ml clotted (red).
  • Sample is allowed to clot at 37 degrees celsius prior to serum separation.
N.A.
Donath Lansteiner 5
  • 10ml clotted (red).
  • Sample is allowed to clot at 37 degrees celsius prior to serum separation.
N.A.
Indirect Antiglobulin Test 5
  • 5ml EDTA sample (preferable)
  • 10ml clotted sample
N.A.
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